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Mechanical vibration induces apoptosis but not necrosis of <t>A431</t> cells. (A, C, and E) Area of apoptotic A431 cells (A A ) normalized to the value for control cells immediately after mechanical vibration (0 h, A) or after 24‐h (B) and 48‐h (C) incubation. (B, D, and F) As described for (A), (C), and (E), except the area of necrotic cells normalized to the control cells (A N ) is shown. Data are presented as the mean ± SD of n = 3. * P < 0.05 by Student's t ‐test
The Human Epidermoid Carcinoma Cell Line A431, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mechanical vibration induces apoptosis but not necrosis of A431 cells. (A, C, and E) Area of apoptotic A431 cells (A A ) normalized to the value for control cells immediately after mechanical vibration (0 h, A) or after 24‐h (B) and 48‐h (C) incubation. (B, D, and F) As described for (A), (C), and (E), except the area of necrotic cells normalized to the control cells (A N ) is shown. Data are presented as the mean ± SD of n = 3. * P < 0.05 by Student's t ‐test

Journal: Engineering in Life Sciences

Article Title: Low‐frequency mechanical vibration induces apoptosis of A431 epidermoid carcinoma cells

doi: 10.1002/elsc.201900154

Figure Lengend Snippet: Mechanical vibration induces apoptosis but not necrosis of A431 cells. (A, C, and E) Area of apoptotic A431 cells (A A ) normalized to the value for control cells immediately after mechanical vibration (0 h, A) or after 24‐h (B) and 48‐h (C) incubation. (B, D, and F) As described for (A), (C), and (E), except the area of necrotic cells normalized to the control cells (A N ) is shown. Data are presented as the mean ± SD of n = 3. * P < 0.05 by Student's t ‐test

Article Snippet: The human epidermoid carcinoma cell line A431, which is considered a good model to study the biomechanical response of cancer cells to extracellular stimuli , was obtained from RIKEN (Wako, Saitama, Japan).

Techniques: Incubation

Mechanical vibration increases glucose consumption by A431 cells. Glucose uptake in culture supernatants was measured between 0 h and 24 h (A) or between 24 h and 48 h (B) after mechanical vibration. Data are presented as the mean ± SD of n = 3

Journal: Engineering in Life Sciences

Article Title: Low‐frequency mechanical vibration induces apoptosis of A431 epidermoid carcinoma cells

doi: 10.1002/elsc.201900154

Figure Lengend Snippet: Mechanical vibration increases glucose consumption by A431 cells. Glucose uptake in culture supernatants was measured between 0 h and 24 h (A) or between 24 h and 48 h (B) after mechanical vibration. Data are presented as the mean ± SD of n = 3

Article Snippet: The human epidermoid carcinoma cell line A431, which is considered a good model to study the biomechanical response of cancer cells to extracellular stimuli , was obtained from RIKEN (Wako, Saitama, Japan).

Techniques:

Mechanical vibration does not increase HMGB1 release from A431 cells. HMGB1 levels in culture supernatants were analyzed by ELISA immediately after 0 h (A), or 24 h (B) and 48 h (C) after vibration. Data are presented as the mean ± SD of n = 3

Journal: Engineering in Life Sciences

Article Title: Low‐frequency mechanical vibration induces apoptosis of A431 epidermoid carcinoma cells

doi: 10.1002/elsc.201900154

Figure Lengend Snippet: Mechanical vibration does not increase HMGB1 release from A431 cells. HMGB1 levels in culture supernatants were analyzed by ELISA immediately after 0 h (A), or 24 h (B) and 48 h (C) after vibration. Data are presented as the mean ± SD of n = 3

Article Snippet: The human epidermoid carcinoma cell line A431, which is considered a good model to study the biomechanical response of cancer cells to extracellular stimuli , was obtained from RIKEN (Wako, Saitama, Japan).

Techniques: Enzyme-linked Immunosorbent Assay